Separation of lymphocytes by electrophoresis under terrestrial conditions and at zero gravity, phase 3

Electrophoretic mobilities (EPM) of peripheral lymphocytes were studied from normal subjects, chronic hemodialysis patients and kidney transplant recipients. A technique to separate B lymphocytes and null cells from non-T lymphocyte preparation was developed. The experiments were designed to determine which subpopulation of the non-T lymphocytes is primarily affected and shows a decreased EPM in chronic hemodialysis patients and kidney transplant recipients

Endosome to Golgi Retrieval of the Vacuolar Protein Sorting Receptor, Vps10p, Requires the Function of the VPS29, VPS30, and VPS35 Gene Products

Mutations in the S. cerevisiae VPS29 and VPS30 genes lead to a selective protein sorting defect in which the vacuolar protein carboxypeptidase Y (CPY) is missorted and secreted from the cell, while other soluble vacuolar hydrolases like proteinase A (PrA) are delivered to the vacuole. This phenotype is similar to that seen in cells with mutations in the previously characterized VPS10 and VPS35 genes. Vps10p is a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases like CPY and PrA, while Vps35p is a peripheral membrane protein which cofractionates with membranes enriched in Vps10p. The sequences of the VPS29, VPS30, and VPS35 genes do not yet give any clues to the functions of their products. Each is predicted to encode a hydrophilic protein with homologues in the human and C. elegans genomes. Interestingly, mutations in the VPS29, VPS30, or VPS35 genes change the subcellular distribution of the Vps10 protein, resulting in a shift of Vps10p from the Golgi to the vacuolar membrane. The route that Vps10p takes to reach the vacuole in a vps35 mutant does not depend upon Sec1p mediated arrival at the plasma membrane but does require the activity of the pre-vacuolar endosomal t-SNARE, Pep12p. A temperature conditional allele of the VPS35 gene was generated and has been found to cause missorting/secretion of CPY and also Vps10p to mislocalize to a vacuolar membrane fraction at the nonpermissive temperature. Vps35p continues to cofractionate with Vps10p in vps29 mutants, suggesting that Vps10p and Vps35p may directly interact. Together, the data indicate that the VPS29, VPS30, and VPS35 gene products are required for the normal recycling of Vps10p from the prevacuolar endosome back to the Golgi where it can initiate additional rounds of vacuolar hydrolase sorting

The VAO Transient Facility

The time-domain community wants robust and reliable tools to enable the production of, and subscription to, community-endorsed event notification packets (VOEvent). The Virtual Astronomical Observatory (VAO) Transient Facility (VTF) is being designed to be the premier brokering service for the community, both collecting and disseminating observations about time-critical astronomical transients but also supporting annotations and the application of intelligent machine-learning to those observations. Two types of activity associated with the facility can therefore be distinguished: core infrastructure, and user services. We review the prior art in both areas, and describe the planned capabilities of the VTF. In particular, we focus on scalability and quality-of-service issues required by the next generation of sky surveys such as LSST and SKA

US VAO Facility for Rapid Transients

The US VAO Facility for Rapid Transients (if funded) will collect and disseminate observations about time-critical astronomical transients, and add annotations and intelligent machine-learning to those observations. The information can be “pushed” to subscribers, who may be either humans or machines that control telescopes. Subscribers can prepare precise “triggers” to decide which events should reach them and their machines, that may be based on the generic event, or on the specific vocabulary of parameters that define a particular type of observation. The system will not be centralized, but rather a set of interoperating nodes with caching. The twin thrusts are automation of process, and discrimination of interesting events

Perceptions of knowledge sharing among small family firm leaders: a structural equation model

Small family firms have many unique relational qualities with implications for how knowledge is passed between individuals. Extant literature posits leadership approach as important in explaining differences in knowledge-sharing climate from one firm to another. This study investigates how leadership approaches interact with family influence to inform perceptions of knowledge sharing. We utilize survey data (n = 110) from owner-managers of knowledge-intensive small family firms in Scotland. Our findings present a choice in leadership intention, contrasting organization-focused participation against family-influenced guidance. Insight is offered on the implications of this leadership choice at both organizational and familial level

Electronic and Magnetic Properties of Electron-doped Superconductor, Sm_{1.85}Ce_{0.15}CuO_{4-delta}

Temperature-dependent magnetization (M(T)) and specific heat (C_p(T)) measurements were carried out on single crystal Sm_{1.85}Ce_{0.15}CuO_{4-delta} (T_c = 16.5 K). The magnetic anisotropy in the static susceptibility, chi {equiv} M/H, is apparent not only in its magnitude but also in its temperature dependence, with chi_{perp} for H{perp}c larger than chi_{parallel} for H{parallel}c. For both field orientations, chi does not follow the Curie-Weiss behavior due to the small energy gap of the J = 7/2 multiplet above the J = 5/2 ground-state multiplet. However, with increasing temperature, chi_{parallel}(T) exhibits a broad minimum near 100 K and then a slow increase while chi_{perp}(T) shows a monotonic decrease. A sharp peak in C_p(T) at 4.7 K manifests an antiferromagnetic ordering. The electronic contribution, gamma, to C_p(T) is estimated to be gamma = 103.2 (7) mJ/moleSmK^2. The entropy associated with the magnetic ordering is much smaller than Rln2, where R is the gas constant, which is usually expected for the doublet ground state of Sm^{+3}. The unusual magnetic and electronic properties evident in M(T) and C_p(T) are probably due to a strong anisotropic interaction between conduction electrons and localized electrons at Sm^{+3} sites.Comment: 5 pages, 5 encapsulated postscript figures, late

Control of atomic currents using a quantum stirring device

We propose a BEC stirring device which can be regarded as the incorporation of a quantum pump into a closed circuit: it produces a DC circulating current in response to a cyclic adiabatic change of two control parameters of an optical trap. We demonstrate the feasibility of this concept and point out that such device can be utilized in order to probe the interatomic interactions.Comment: 5 pages, 4 figures, uses epl2.cls, revised versio

Circle talks as situated experiential learning: Context, identity, and knowledgeability in \u27learning from reflection\u27

This article presents research that used ethnographic and sociolinguistic methods to study ways participants learn through reflection when carried out as a “circle talk.” The data indicate that participants in the event (a) invoked different contextual frames that (b) implicated them in various identity positions, which (c) affected how they could express their knowledge. These features worked together to generate socially shared meanings that enabled participants to jointly achieve conceptualization—the ideational role “reflection” is presumed to play in the experiential learning process. The analysis supports the claim that participants generate new knowledge in reflection, but challenges individualistic and cognitive assumptions regarding how this occurs. The article builds on situated views of experiential learning by showing how knowledge can be understood as socially shared and how learning and identity formation are mutually entailing processes